Isolation of glucose-containing high-mannose glycoprotein core oligosaccharides.
نویسندگان
چکیده
منابع مشابه
High-mannose oligosaccharides from human Tamm-Horsfall glycoprotein.
The present communication reports the occurrence of high-mannose oligosaccharides on Tamm-Horsfall glycoprotein prepared from human pooled urine. The Pronase digest of the glycoprotein was fractionated by gel filtration and a high-mannose glycopeptide species was separated from complex-type glycopeptides. When high-mannose glycopeptides were digested with endo-beta-N-acetylglucosaminidase H, fo...
متن کاملFormation of lipid-bound oligosaccharides containing mannose. Their role in glycoprotein synthesis.
Incubation of GDP-[(14)C]mannose with liver microsomes and magnesium ions led to the formation of dolichol monophosphate mannose and of other acidlabile compounds that contain oligosaccharides. Formation of these compounds was enhanced by addition of an acceptor lipid in the same fractions of DEAE-cellulose chromatography where bound dolichol is found. Alkaline treatment of the oligosaccharides...
متن کاملOligosaccharides containing glucose and mannose in glycoproteins of the thyroid gland.
The glucose-containing oligosaccharides formed by calf thyroid slices incubated with radioactive glucose were studied. A compound soluble in chloroform/methanol/water, 1:1:0.3 (vol/vol), was found that was indistinguishable from the previously described glucose-containing dolichyl diphosphate oligosaccharide formed by liver microsomes. Glycopeptides were prepared by treating the glycoproteins w...
متن کاملN-Linked High Mannose-type Oligosaccharides
Incubation of Crithidia fasciculata cells with [U-’“C] glucose led to the synthesis of Man-P-dolichol but not of Glc-P-dolichol. The main and largest dolicho1-P-Plinked oligosaccharide formed was Man7GlcNAcz whether labeling was performed in 5 mM sodium pyruvate or 5.5 mM glucose. The protein-linked, endo-j3-N-acetylglucosaminidase H-sensitive oligosaccharides isolated from mature glycoprote...
متن کاملIdentification of a novel mechanism for the removal of glucose residues from high mannose-type oligosaccharides.
The role of glucosylated oligosaccharides in the biogenesis of the glycoprotein (G protein) of vesicular stomatitis virus was studied in PhaR2.7, a mouse lymphoma cell line deficient in glucosidase II activity. As expected, the great majority of cell-associated G protein remained glucosylated in PhaR2.7, and the G protein was rapidly deglucosylated in BW5147, the parental cell line. Despite the...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1984
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.81.20.6340